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Light Microscopy Slide Preparation

Materials Needed

Instructions to prepare stain.

  1. Mix ~75mL distilled water with ~75mL 100% ethyl alcohol (ETOH). 
  2. Mix in ~0.5g of Safranin O stain.

Slide preparation instructions:

  1. Supplies needed to prepare slides. mvc-008f.JPG (17939 bytes)
  2. Slide with glycerin and stain. mvc-010f.JPG (17981 bytes)mvc-011f.JPG (17520 bytes)
  3. Placement of coverslip on slide. mvc-012f.JPG (17203 bytes)
  4. Coverslip on slide.mvc-013f.JPG (16865 bytes)
  5. Sealing the slide with nail polish.. mvc-014f.JPG (19316 bytes)mvc-015f.JPG (18608 bytes)mvc-016f.JPG (19209 bytes)mvc-017f.JPG (18276 bytes)
  6. Slide with label. mvc-009f.JPG (19752 bytes)
  7. Placing the slide in the slide box. mvc-018f.JPG (21579 bytes)mvc-019f.JPG (22535 bytes)
  8. Slide boxes. mvc-020f.JPG (21026 bytes)